| 학회 |
한국공업화학회 |
| 학술대회 |
2008년 가을 (11/12 ~ 11/14, ICC 제주) |
| 권호 |
12권 2호 |
| 발표분야 |
콜로이드계면화학 심포지엄 |
| 제목 |
Biosensors based on the selective aggregation of nanoparticles |
| 초록 |
We have performed the detection of specific DNA sequences using selective aggregation of unmodified gold nanoparticles by the hybridization of oligonucleotide. Oligonucleotide sequences were designed to detect the signal-induced proliferation-associated gene 1 (Sipa1) since Sipa1 polymorphism is known to be associated with metastatic process. To monitor selective aggregation of gold nanoparticles, we used the UV-vis absorption spectroscopy, quasi-elastic light scattering (QELS) and zeta potential measurement. We were able to selectively aggregate gold nanoparticles for the perfectly matched DNA under optimal salt concentration, which enables a detection of perfectly matched DNA from the single point-mutated one. This suggests a novel design principle for a rapid detection of the DNA sequences by controlling the electrostatic interactions between gold nanoparticles. |
| 저자 |
이강택1, 김태훈1, 이윤희1, 이소영2, 주상우3
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| 소속 |
1연세대, 2서울대, 3숭실대 |
| 키워드 |
Biosensors; gold nanoparticle; aggregation; DNA
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| E-Mail |
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