| 학회 |
한국화학공학회 |
| 학술대회 |
2012년 가을 (10/24 ~ 10/26, 부산 BEXCO) |
| 권호 |
18권 2호, p.1896 |
| 발표분야 |
생물화공 |
| 제목 |
Construction of a sucrose-utilizing Escherichia coli K-12 strain by β-fructofuranosidase introduction and its application for threonine production |
| 초록 |
Sucrose is one of the most promising carbon sources for industrial fermentation. Here, we report a practical example of developing sucrose-utilizing platforms using Escherichia coli K-12. In this study, we constructed E. coli K-12 strains capable of uptaking sucrose by introducing β-fructofuranosidases. Analyses of the underlying mechanism revealed that sucrose was hydrolyzed into glucose and fructose in the extracellular space and both liberated hexoses could be transported by their respective uptake systems in E. coli K-12. This system was introduced into the engineered L-threonine production strain of E. coli K-12 to produce threonine from sucrose. [This work was supported by the Technology Development Program to Solve Climate Changes from National Research Foundation of Korea (Development of systems metabolic engineering platform technologies for biorefineries; NRF-2012-C1AAA001-2012M1A2A2026556) funded by the Ministry of Education, Science and Technology. Further supports by the World Class University Program (R32-2008-000-10142-0) of the MEST were appreciated.] |
| 저자 |
송찬우1, 이정욱2, 최 솔1, 이상엽1
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| 소속 |
1KAIST, 2KAIST / Boston Univ. |
| 키워드 |
sucrose; biorefinery; threonine
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| E-Mail |
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| 원문파일 |
초록 보기 |
