| 초록 |
In this study, we developed angiogenesis model to examine endothelial sprouting behavior and metabolic expression level under microgravity. This model was assembled with human umbilical vein endothelial cells (HUVECs) and the cells were cultured on the surface of dextran microcarriers, and they were cultured in a rotary cell culture systems (RCCS) to stimulate under microgravity. After exposure to simulated microgravity, dextran microcarriers coated HUVECs were embedded fibrin gel, and then, we observed sprouting of HUVECs. In addition, by applying the LC-MS-based quantitative method, we monitored changes in the metabolic expression level in endothelial cells stimulated under the microgravity. Taken together, the results of this study will be highly useful in designing various angiogenesis models to recapitulate and further manipulate the tissue of interest in space environments. |
