| 초록 |
The lignin peroxidase H2 gene was isolated from P. chrysosporium. The recombinant LiP H2 was similar to that of horseradish peroxidase and lignin peroxidase, indicating that the molecular weight of recombinant LiP was about to be 38Kda. We performed cloning into pYD vector for yeast cell surface display of lignin peroxidase H2 gene. In result by rapid screening method, 5 colonies were expressed Lignin peroxidase H2 at high level. Supernatants were not changed color, but washed cells were changed color from white to red. 2,4 - DCP degradation activity of surface displayed LiP was shown 21.5% - 25.1%. In this aspect, the yeast surface displayed lignin peroxidase could utilize the degradation of xenobiotics or pollutants, aromatic compound. |
