| 초록 |
The basic concept described for guided bone regeneration (GBR) involves placement of barrier membrane to create a separated space around bone defect to protect the invasion of connective tissue. Collagen is the most abundant protein in mammals and a variety of collagen-based scaffolds have been demonstrated by tissue engineering applications. In this study, we fabricated double-layered collagen membrane (DLCM) using electrospinning, which should be futher cross-linked by glutar- aldehyde vapor. Nanofiber diameter was around 395nm, which increased to 603nm after cross-linking. The DLCM was subject to detailed analysis by SEM, ATR-FTIR, and Tensile strength. In addition, cyto-compatibility of DLCM was examined by MTT assay and SEM observation, resulting that no cytotoxicity toward growth of Osteoblast and Fibroblast. Then Micro structure of DLCM showed no invasion connective tissue. In conclusion, the electrospun DLCM will potentially provide better environment for bone regeneration. |
