| 초록 |
Low molecular water soluble chitosan(LMWSC) has both reactive amino and hydroxyl groups that can be used to chemically alter its properties under mild reaction conditions. LMWSC is ease of soluble in neutral aqueous solution. Its advantage is ease of modification, useful as a gene or peptide drug carriers, and drug carriers. All-trans retinoic acid (atRA) is effective in the treatment of epithelial and hematological malignancies such as breast cancer, head and neck cancer, ovarian adenocarcinoma, and acute promyelocytic leukemia (APL). Core-shell type nanoparticles has advantages compared to plain nanoparticles, i.e. hydrophilic shell protect drug encapsulated core from attack of reticuloendothelial system (RES). In this study, we synthesized graft compolymers composed of (LMWSC) and poly(ethylene gycol) (abbreviated as WSCP) to prepare core-shell type nanoparticles based on polyelectrolyte complexes between LMWSC backbone and anionic drug. Nanoparticulate polyelectrolyte complexes were formed through ultrasonication. To form core-shell type nanoparticles with WSCP and atRA, various M.W. of WSCP were used and showed ability to form nanoparticles at almost of the conditions. Particle sizes of chitosan-drug polyelectrolyte complexes was 50 - 800 nm according to the M.W. of chitosan and drug amount. Loading efficiency of drug into chitosan polyelectrolyte complexes was over 60 % (w/w) at all of the formulation. This drug loading efficiency was higher than other types of drug carriers such as liposomes, conventional nanoparticles, and lipid nanoparticles. |
