| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 봄 (04/08 ~ 04/10, 대전컨벤션센터) |
| 권호 |
40권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Thiolated Akt1 siRNA nanoparticle for efficient anti-cancer gene |
| 초록 |
In cancer cells, Akt1 protein kinase is one of the key components that is responsible for cancer cell proliferation and apoptotic inhibition. Silencing of Akt1 protein using siRNA in many cancer cells has shown reduction in cell proliferation and induction of apoptosis. Hence for the current study, we designed a nanoparticle using disulfide crosslinked polyethylenimine (ssPEI) conjugated with thiolated Akt1 siRNA (SH-Akt siRNA) through disulfide bonding. Disulfide bonding between SH-Akt siRNA and ssPEI has enhanced the complexation and reduced surface charge with average particle sizes even at lower N/P ratio compared to the branched PEI 25k/SH-Akt siRNA polyplex. In in-vitro condition, the increase in cellular uptake and siRNA release from nanoparticle in the cytoplasm has led to reduction in proliferation as well as induction of apoptosis in CT-26 mice tumor model. Overall, the Akt1 silencing using ssPEI/SH-Akt siRNA have reduced the tumor volume and size in mouse tumor model. |
| 저자 |
산토시, Cherukula Kondareddy, 박인규
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| 소속 |
전남대 |
| 키워드 |
siRNA; Akt1; Colon cancer; Polyethylenimine; Apoptosis; Proliferation
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| E-Mail |
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