초록 |
Water-soluble chitosan (WSC) is derived from chitosan and has low molecular weight. Since chitosan is only soluble in acidic water, WSC has advantages such as excellent solubility in deionized water, ease of chemical modification, altered functionality and enhanced bioactivity. Especially, WSC is regarded to have enhanced anti-carcinogenecity, anti-microbial activity, and, especially, enhanced mucosal delivery of bioactive agents. In this study, we prepared photosensitizer-incorporated WSC nanoparticles through ion complex formation between WSC and Ce6 as photosensitizer. Photosentizer-incorporated WSC (ChitoCe6) has small particle sizes less than 100 nm at TEM and particle size. ChitoCe6 enhances delivery ability to HCT116 human colon carcinoma cells rather than Ce6 itself. Also, ChitoCe6 generated ROS higher than Ce6 itself despite of anti-oxidant properties of WSC. ChitoCe6 enhances phototoxicity against tumor cells and induced apoptosis/necrosis of tumor cells. We suggest WSC nanoparticles is promising vehicles for photosensitizer delivery. |