| 학회 |
한국공업화학회 |
| 학술대회 |
2016년 가을 (10/26 ~ 10/28, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
20권 2호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Application of fluorescence-activating and absorption-shifting tag for multiplexed live cell imaging |
| 초록 |
Fluorescent labeling of proteins has been greatly important for bioimaging to study complex molecular mechanisms in cells. The yellow fluorescence-activating and absorption-shifting tag (Y-FAST) is 14-kd protein which by addition of a fluorogen can be rapidly and reversibly activated. However, an effective strategy for multiplexed imaging while allowing temporal control has been lacking. We have developed a multiplexed version of the Y-FAST protein by synthesizing different chromophores that allow fluorescence activation and spectral shifts in various wavelengths. The effects of binding of the fluorogens to Y-FAST were characterized using the purified protein and live cells expressing the protein. This system can be potentially applied for tagging various target proteins for multiplexed imaging in live cells for studies on drug binding, function, and signaling. |
| 저자 |
김주강, 정현정
|
| 소속 |
한국과학기술원 |
| 키워드 |
Fluorescent labeling; Y-FAST; multiplex imaging
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| E-Mail |
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