| 초록 |
A new technique for the density control of self-assembled monolayers of metalloproteins including ferredoxin and cytochrome c onto metal substrates were developed using a zwitterionic surfactant. The elution ability of the selected surfactant, CHAPS ( 3 - [ ( 3 - cholamidopropyl ) dimethylammonio ] - 1 - propanesulfonate ), enabled us to segregate protein aggregates nonspecifically adsorbed on the metal substrates, which has been a long-term problem in the fabrication of biomolecular electronic devices. The high-resolution AFM images of self-assembled protein monolayers from the surface of metal substrates with CHAPS treatment clearly shows that the size of protein clusters is on the order of a few molecules of proteins. Whereas the sizes of protein aggregates on the same substrate without CHAPS treatment were measured to be about 200nm. The electrochemical property of self-assembled protein monolayer on gold surface was confirmed to remain intact by means of the cyclic voltammetry measurements. The present technique would be useful to the preparation of self-assembled monolayer with diverse biomolecules.
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