| 초록 |
Small interfering RNA (siRNA) has selectively recognize and cleave the target mRNA. Due to their innate physiochemical characteristics, however, the systemic delivery of naked siRNA fails to achieve therapeutically relevant gene silencing. To this end, a variety of cationic polymers and lipids have extensively studied for efficient siRNA delivery. In this study, we introduced nucleic acid aptamers on siRNA, which can enhance the cellular uptake. Aptamers sequences are directly synthesized with siRNA without coupling chemistry. In addition, DNA nanostructures were employed for delivering of aptamer-siRNA (apt-siRNA). DNA nanostructures can regulated the density and location of apt-siRNA in small area and can alter the Pharmacokinetics in vivo. apt-siRNA showed enhanced uptake efficiency. Also, when apt-siRNA conjugates was hybridized with holiday DNA nanostructure, cellular uptake efficiency and target gene knockdown efficiency increased than that of only apt-siRNA. |
