| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 봄 (04/11 ~ 04/12, 대전컨벤션센터) |
| 권호 |
38권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
pH – responsive organic nano-indicator tailored by pseudo metal for trafficking endolysosomal compartment |
| 초록 |
Intracellular pH plays a crucial role in cell biology since proton gradients underlie the function of organelles such as endosome, lysosomes and mitochondria. Therefore, observation of proton gradients in cell components is essential. One promising approach is the assessment of nanoparticle based on polyaniline (PANI) that exhibited convertible transition state by proton which had the feasibility as observer of intracellular pH. Here, we synthesized silica coated PANI nanoparticles (PSIsNIs) as indicator. First, hetero structure of Fe3O4/MnO (IsNIs) was synthesized and coated with silica (SIsNIs). Subsequently, PSIsNIs were formed by IsNIs as oxidant and shifting agent of transition state from pH3 to pH6. Finally, Cy3 and Cy7 as pH insensitive dye was adsorbed and PEGylated in PSIsNIs, respectively (FPSIsNIs). To assess the potential of FPSIsNIs as indicator, we investigated morphology, optical properties, and we evaluated quenching ability according to proton gradient in vitro. |
| 저자 |
최은비, 최지혜, 장은지, 김현욱, 강병훈, 김명훈, 서진석, 허용민, 함승주
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| 소속 |
연세대 |
| 키워드 |
pH; cell compartment; polyaniline; quenching
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| E-Mail |
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