| 초록 |
In this study, we report polymer nanoparticles (NPs) that can induce enhanced immune response in dendritic cell (DC)-based cancer immunotherapy, by the combination delivery of tumor antigen and small interference RNA (siRNA) for immunosuppressive gene to dendritic cells. Multifunctional PLGA NPs that can deliver tumor antigen and siRNA for immunosuppressive SOCS1 genes to DCs simultaneously were fabricated by the emulsion solvent evaporation method. We have found that the encapsulation efficiency of small-sized and hydrophilic SOCS1 siRNA into hydrophobic PLGA matrix was drastically enhanced by the help of tumor model antigen such as ovalbumin (OVA). The knockdown of SOCS1 in BMDCs by PLGA (OVA/SOCS1 siRNA) NPs enhanced pro-inflammatory cytokine, interleukin 6 (IL-6), expression. Additionally, PLGA (OVA/SOCS1 siRNA) NPs-treated BMDCs could elicit an immune response through cross-presentation in OVA-specific CD8 T cells that express interleukin 2 (IL-2) cytokine. |
