| 초록 |
In this study, the phosphorylated 2-hydroxychalcone (HCAP) fluorescent probe was conjugated with coatable catechol-bromododecane quaternized poly(vinylpyrrolidone) (C/C12-PVP) polymer to obtain C/C12-PVP conjugated with HCAP (HCAP-PVP) for detection and antibacterial activity. When bacteria were introduced into HCAP-PVP, the phosphate group of HCAP was degraded by endogenous alkaline phosphatase (ALP) enzyme and the greenish-yellow emission of the probe was shifted to red. Hence, in the presence of quaternary ammonium of dodecane, HCAP-PVP showed an effective antibacterial activity in both solid and aqueous states. Therefore, this sensor can be used as a simple and fast enzymatic biosensor for rapidly detecting and killing bacteria with high sensitivity. |
