| 초록 |
Global DNA methylation is in the spotlight as a treatment and diagnostic strategy for several severe diseases. However, conventional methods entail laborious work, high costs, and high concentrations of DNA sample. We developed a new method based on surface-enhanced Raman spectroscopy (SERS) to detect global DNA methylation. This was chosen due to the two reasons: (i) genomic DNA has many neighbouring methylcytosine (mCpG) sites in CpG islands, and (ii) anti-mCpG immunogold colloids are coupled to amplify plasmon coupling when they bind to the mCpG sites on the genome. Global DNA methylation was detected via SERS in a coupled gold nanoparticle–silver nanowire (AgNW) and a simple glass-based system. DNA samples were absorbed onto AgNW surface and a microscopic glass, and immunogold colloids were then used to detect the methylation status. The detection limits for global methylated DNA on the platform of glass and the silver nanowire were 195 fg ml−1 and 18 fg ml−1, respectively. |
