| 초록 |
In tissue engineering, the ideal scaffold should mimic the structure and function of the native extracellular matrix (ECM). In particular, scaffolds should act as a depot for various cytokines and growth factors for the control of cell and tissue responses. In this study, heparin-immobilized nanofibrous scaffolds, which have various volumes, based on polycaprolactone and gelatin crosslinked with genipin were fabricated to release bFGF in a sustained manner. Heparin was covalently conjugated onto the surface of nanofibrous scaffolds and bFGF was then specifically immobilized on the scaffolds. The amount of immobilized bFGF was increased with increasing the volume of nanofibrous scaffold. Sustained release of bFGF was successfully achieved for over 2 months. The bFGF released from the nanofibrous scaffolds maintained its bioactivity and the in vitro proliferation of human umbilical vein endothelial cells was increased as the amount of bFGF was increased in the nanofibrous scaffold. |
