| 학회 | 한국화학공학회 |
| 학술대회 | 2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 | 10권 1호, p.388 |
| 발표분야 | 생물화공 |
| 제목 | Effect of n-alkanethiolates on the formation of self-assembled straptavidin-biotin monolayer on a gold surface |
| 초록 | In an attempt to find an optimum condition for immobilization of streptavidin, self-assembled monolayers (SAMs) of biotin-terminated long-chain n-alkanethiolates (HS(CH2)nX, where n = 11, 12, and 16 ) and their mixtures with 11-mercaptoundecanol from solution onto the gold surfaces were prepared and characterized by a broad range of surface analytical techniques including optical ellipsometry, contact angle goniometry, X-ray photoelectron spectroscopy (XPS), and scanning tunneling microscopy (STM). Studies on wettability, composition, and film thickness of these SAMs show that the packing density of biotin-modified SAMs is affected by a number of alkyl chains of n-alkanethiols, especially even/odd numbers of alkyl chains. The film thickness and surface images of streptavidin binding to biotin-modified mixed SAMs formed from different mole fraction of solution indicate that the specific binding between streptavidin and biotin is maximized at very low mole fraction of biotin-terminated n-alkanethiolates in solution. * 본 연구는 환경부 차세대 핵심 환경기술개발사업의 일환으로 수행되었습니다. |
| 저자 | 김대진, 이종범, 구기갑 |
| 소속 | 서강대 |
| 키워드 | self-assembled monolayers; streptavidin-biotin; alkanethiols |
| VOD | VOD 보기 |
| 원문파일 | 초록 보기 |
