| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Carbon flux rebalancing for efficient conversion of 3-hydroxypripionate from acetate in engineered Escherichia coli |
| 초록 |
Acetate is a potential feedstock for microbial processes that produce diverse value-added chemicals. In this study, we produced 3-hydroxypropionic acid from acetate with engineered Escherichia coli. For efficient conversion of acetate to 3-HP, we initially introduced heterologous mcr (encoding malonyl-CoA reductase) from Chloroflexus aurantiacus. Then, the acetate assimilating pathway and glyoxylate shunt pathway were activated by overexpressing acs (encoding acetyl-CoA synthetase) and deleting iclR (encoding the glyoxylate shunt pathway repressor). Because a key precursor malonyl-CoA is also consumed for fatty acid synthesis, we decreased carbon flux to fatty acid synthesis by adding cerulenin. Subsequently, we found that inhibiting fatty acid synthesis dramatically improved 3-HP production (3.00 g/L of 3-HP from 8.98 g/L of acetate, 44.6% of the theoretical maximum yield). The results indicated that acetate can be used as a promising carbon source for microbial processes. |
| 저자 |
이지연, 차상학, 이지훈, 강채원, 이건민, 임현규, 정규열
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| 소속 |
포항공과대 |
| 키워드 |
3-Hydroxypriopionic acid; Acetate; Malonyl-CoA; Escherichia coli
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| E-Mail |
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