| 학회 |
한국화학공학회 |
| 학술대회 |
2019년 가을 (10/23 ~ 10/25, 대전컨벤션센터) |
| 권호 |
25권 2호, p.1494 |
| 발표분야 |
생물화공 (Biochemical Engineering) |
| 제목 |
Production of 3-hydroxy propionic acid from levulinic acid using recombinant Pseudomonas putida KT2440 |
| 초록 |
Levulinic acid (LA) is a five γ-keto carbon acid that can be easily obtained through acid hydrolysis without enzyme reaction from cellulose. 3-Hydroxy propionic acid (3-HP) is a monomer of the biodegradable polymers that can be replaced petrochemistry based polymers. In this study, LA is proposed as a novel carbon source for 3-HP production by using Pseudomonas putida, gram negative soil bacteria. According to the complete annotated genome information, P. putida contains the metabolic pathway of LA. In M9 minimal media with 75mM of LA, P. putida grew to OD6008.15 without growth inhibition. Because LA is catabolized to propionyl-CoA and acetyl-CoA, butyryl-CoA dehydrogenase, enoyl-CoA hydratase and 3-hydroxy isobutyryl-CoA hydrolase from P. putida were overexpressed to convert propionyl-CoA to 3-HP. After that, prpC gene, 2-methylcitrate synthase, was deleted to increase the yield of 3-HP by blocking the methylcitrate cycle. Finally, with these genetic modifications, the production of 3-HP from LA is confirmed. The results of this study suggest that P. putida is a novel candidate that can biosynthesize 3-HP from LA as a sole carbon source. |
| 저자 |
손지희1, 양정모1, 조숙형2, 연영주3, 이진원1
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| 소속 |
1서강대, 2C1 리파이너리 사업단, 3강릉원주대 |
| 키워드 |
생물화학공학 |
| E-Mail |
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| 원문파일 |
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