| 학회 |
한국화학공학회 |
| 학술대회 |
2011년 가을 (10/26 ~ 10/28, 송도컨벤시아) |
| 권호 |
17권 2호, p.1455 |
| 발표분야 |
생물화공 |
| 제목 |
Enzymatic synthesis of 2-butanone from 4-oxopentanoic acid by acetoacetate decarboxylase from Clostridium acetobutylicum |
| 초록 |
2-Butanone is a widely used low-boiling solvent in various industries and currently is produced by the dehydrogenation of secondary butanol or the direct oxidation of n-butene.Herein, we presents a renewable synthesis of 2-butanone from 4-oxopentanoic acid (4OPA), which is produced from cellulosic biomass and considered as an important building block for value-added chemicals, via enzymatic decarboxylation. Acetoacetate decarboxylase (AADC) from Clostridium acetobutylicum was employed as the biocatalyst and expressed in E.coli with a 6xHis tag. Permeabilized whole cells of E. coli expressing AADC converted 8.8% of 4OPA into 2-butanone. The purified enzyme converted 26.1% of 4OPA into 2-butanone. The kinetic parameters and stability of the enzyme were investigated. We also propose the catalytic mechanism of 4OPA to 2-butanone via enzymatic decarboxylation. |
| 저자 |
민경선1, 이회석2, 엄영순3, 상병인4, 유영제1
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| 소속 |
1Clean Energy Research Center, 2KIST, 3Graduate Program in Bioengineering, 4Seoul National Univ. |
| 키워드 |
4-oxopentanoic acid; 2-butanone; acetoacetate decarboxylase
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| E-Mail |
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| 원문파일 |
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