| 초록 |
A novel hydrogel system using oligo(amido amine/β-amino ester) (OAAAE) was synthesized for controlled release of insulin. OAAAE was prepared by the Michael-addition oligomerization of the secondary amine groups of 4,4-trimethylene dipiperidine with the vinyl groups of 1,8-octylene diacrylamide and 1,6-hexanediol diacrylate. The formed oligomer was characterized by 1H NMR and gel permeation chromatography. OAAAE in aqueous solution (20 wt%) underwent a sol-to-gel transition with increasing pH and a gel-to-sol transition with increasing temperature. The degradation of the hydrogel, influence of insulin on gel phase and gel strength, in vitro cytotoxicity of OAAAE, in vitro and in vivo release of insulin from the complex hydrogel were investigated. Furthermore, the in vivo release profile of insulin from the complex hydrogel was compared with that from the neutral hydrogel. |
