| 학회 |
한국고분자학회 |
| 학술대회 |
2002년 봄 (04/12 ~ 04/13, 서울대학교) |
| 권호 |
27권 1호, p.269 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Effect of surface energy and collagen immobilization on the adhesion of endothelial cells on hydrophilic/ hydrophobic IPNs |
| 초록 |
When an engineered tissue is constructed, the adhesion of target cells on synthetic materials is first event of the process, which is highly affected by the surface properties of the materials. In this study, hydrophilic polyurethane (PU)/ hydrophobic polystyrene (PS) interpenetrating polymer networks (IPNs) were prepared with changing the surface energy. The surface energy of PU/ PS IPNs was controlled by changing the mold material used during the synthesis of the IPNs. The result of the contact angle measurement and ESCA showed that the surface energy of the IPNs varied from 28.4 mJ/m2 to 37.7 mJ/m2, and O/C ratio varied from 0.05 to 0.19. After immobilization of the collagen, the human umbrical vein endothelial cells (HUVECs) were made to adhere to the IPN surfaces. The adhesion of the HUVECs on the IPNs was observed by using SEM, and the percentage of the adhesion was calculated by using hemacytometer. The amount of the adhered HUVECs was increased by the immobilization of the collagens on the IPNs. The percentage of the adhesion of HUVECs was maximized on the collagen-immobilized IPN surfaces of which the surface energy was about 30.0 mJ/m2, which indicated that the immobilization of the collagens became very effective on the IPNs with a specific surface energy. |
| 저자 |
김종훈1, 이유경2, 윤성수, 박태관*, 김성철
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| 소속 |
1한국과학기술원 화학공학과, 2*한국과학기술원 생물과학과 |
| 키워드 |
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| E-Mail |
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