| 초록 |
The characterization based on size exclusion chromatography (SEC) cannot do the job due to its sensitivity on the chain size and relatively low resolution. In this study, we demonstrate the use of the temperature gradient interaction chromatography (TGIC) to characterize the partially deuterated comb shaped polymers. Normal phase TGIC can separates the comb polymer according to the molecular weight quite well. We also found that a significant isotope effect exits in the reverse phase TGIC retention between deuterataed and hydrogenous polymers. Taking advantage of the isotope sensitivity of RPLC, the comb polymers are cross fractionated by NPLC and RPLC, and the two dimensional mapping with respect to the backbone chain length and the number of side-chains is fully established. |
