| 초록 |
As hydrocarbons are currently used as liquid fuel, the bacterial conversion of biomass to hydrocarbons such as fatty acids has been a point of interest. Recently, a recombinant strains for the improvement of the biohydrocarbon accumulation in the cells has been reported. E. coli MG1655 encoding all enzymes that catalyze key steps in the synthesis of fatty acids was obtained. In the present work, a process is proposed for the recovery of fatty acids from Recombinant E. coli MG1655. After the growth of the recombinant E.coli, the cells were disrupted by high pressure homogenizer for obtaining intracellular fatty acids. Then, the resulting solutions were extracted by several solvents. Finally, the optimum conditions for the recovery process were obtained and the effects of these operating parameters were discussed. |
