| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 가을 (10/11 ~ 10/12, 일산킨텍스) |
| 권호 |
32권 2호 |
| 발표분야 |
생체모방형 고분자재료(의료용 고분자 부문위원회) |
| 제목 |
Osteogenic differentiation of bone marrow derived human mesenchymal stem cells(hMSCs) on electrospun poly(L-lactide-co-ε-caprolactone)(PLCL) and gelatin composite nanofiber scaffolds |
| 초록 |
In tissue engineering, it is important to develop scaffolds that can be functional to control morphogenic response of progenitor cells and to direct desirable regeneration. In this study, we fabricated three-dimensional nanofibrous scaffolds by electrospinning polymer blends of poly(L-lactide-co-ε-caprolactone)(PLCL) and gelatin to modulate differentiation of human mesenchymal stem cells (hMSCs). We hypothesized that the combination of natural and synthetic polymers may provide an ideal substrate for hMSCs culture. hMSCs were seeded on four types of scaffold and induced to differentiate along osteogenic lineages. We examined gene expression of osteogenic markers, calcium deposition, and alkaline phospatase activity. Cells seeded on PLCL/gelatin blend scaffold showed higher level of ALP activity and calcium deposition than those seeded on PLCL scaffold. These results suggest that PLCL/gelatin blend nano-fibrous scaffolds may provide biomimetic ECM environment for tissue regeneration. |
| 저자 |
임내균, 정성인, 고은경, 신흥수
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| 소속 |
한양대 |
| 키워드 |
MSC; scaffold; PLCL; gelatin; osteogenic; differentiation; electrospun
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| E-Mail |
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