| 초록 |
DNA-templated silver nanoclusters (DNA-AgNCs) have emerged as promising material for sensing, bio-labelling, and bio-imaging by its fluorogenic property. Herein, we developed two different types of DNA-AgNCs systems for the detection of nitric oxide (NO) and potassium ion (K+). NO detection was accomplished using C12 sequence by NO-induced deamination of cytosine. Mutation of cytosine to deoxyuridine destabilized the DNA-AgNCs structure, resulting in quenching fluorescence intensity. On the other hand, thrombin binding aptamer was used as both template of AgNCs and binding probe of K+ for sensing. The fluorescence emission of DNA-AgNCs probe was diminished proportionally to the amount of K+ detected, which transformed AgNCs-adapted structure into G-quadruplex. The characteristics of these probes were analyzed by UV absorption, fluorescence emission, and TEM image. We expect that these system enable to open a new horizon for highly sensitive and selective detection systems. |
