| 학회 |
한국공업화학회 |
| 학술대회 |
2014년 가을 (11/12 ~ 11/14, 엑스코(대구)) |
| 권호 |
18권 2호 |
| 발표분야 |
(생물공학)Advances in nanobiotechnology |
| 제목 |
A GFP variant exclusively deposited to fluorescent protein nanoparticles |
| 초록 |
Inclusion bodies (IBs) were generally considered to be inactive protein deposits and did not hold any attractive values in biotechnological applications. Recently, some IBs of recombinant proteins were confirmed to show their functional properties such as enzyme activities, fluorescence, etc. Such biologically active IBs are not commonly formed, but they have great potentials in the fields of biocatalysis, material science an nanotechnology. Here, we introduce a DL4, a deletion variant of green fluorescent protein which forms active intracellular aggregates. The intrinsic property of DL4 to form aggregates in vivo was exploited to produce fluorescent protein particles with different sizes. It is expected that DL4 IBs can be used as fluorescent biomaterials. |
| 저자 |
Govindan Raghunathan, Sriram Sokalingam, Ganapathiraman Munussami, 이선구
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| 소속 |
부산대 |
| 키워드 |
Green fluorescent protein; Active inclusion bodies; Protein aggregation; Fluorescent particle
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| E-Mail |
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