| 초록 |
Production of platform chemicals from abundant biomass with high productivity and yield is a key of bio-refinery. Unfortunately, microbes slowly utilize biomass-derived sugars and produce unsatisfied amount of desired biochemical. Thus, redesign of microorganisms is necessary to achieve enhanced product formation. In this study, we engineered Escherichia coli to produce cadaverine with fast utilization of galactose through implementation of reconstructed pathways. The expression cassettes of genes coding metabolic enzymes were rationally designed for maximum catalytic activity through employing synthetic promoters, 5’ UTRs. In addition, competing pathways were inactivated to increase carbon flux. Finally, the engineered strain produced 8.80 g/L cadaverine with high yield (0.170 g/g) and productivity (0.293 g/L/h) during fed-batch cultivation. This is the first report to produce cadaverine from galactose, moreover, the yield was the highest ever reported including the yield on glucose. |
