| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
The translocation of elastin-like polypeptide for gene delivery |
| 초록 |
Efficient gene delivery would significantly impact in clinical therapies, diagnostics and basic science research. For non-viral vector translocation, biomaterials are a critical component for the development of efficient delivery systems. In the presentation, elastin-like polypeptide (ELP) was used for a vehicle for specific cellular uptake because of its inverse transition temperature (Tt) and biocompatibility. Thermoresponsive elastin-like polypeptide (ELP) was synthesized by recombinant DNA technique. Inverse transition temperature of stimuli-responsive bioactive ELP was characterized using UV spectrometer. The phase transition resulted in changes of binding affinity for plasmid DNA. For improving cellular uptake efficiency, ELP fused to cell penetrating peptide (CPP) was constructed. Cellular uptake efficiency was investigated through expression level of green fluorescent protein. |
| 저자 |
나경아1, 현진호1, 김옥진2, 김경철2, 정재연1, 신병철3
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| 소속 |
1서울대, 2바이오시스템소재학부, 3한국화학(연) |
| 키워드 |
elastin-like polypeptide (ELP); inverse phase transition; gene delivery
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| E-Mail |
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