| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
Target Specific Intracellular Delivery of siRNA Complexed with PEI-HA Conjugate |
| 초록 |
A novel target specific small interfering RNA (siRNA) delivery system was developed using polyethylenimine (PEI) - hyaluronic acid (HA) conjugate. The siRNA / PEI-HA complex appeared to be formed by electrostatic interaction between the negatively charged siRNA and the positively charged PEI of PEI-HA conjugate. When B16F1 and HEK-293 cells were treated with fluorescein isothiocyanate (FITC) labeled siRNA/PEI-HA complex, B16F1 cells, with a lymphatic vessel endothelial hyaluronan receptor-1 (LYVE-1), showed higher green fluorescence than HEK-293 cells due to the HA receptor mediated endocytosis of the complex. In addition, the gene silencing effect was reduced in the presence of free HA in the transfection medium, showing that the conjugates were selectively taken up by B16F1 cells via receptor mediated endocytosis. Furthermore, intratumoral injection of anti-VEGF siRNA/PEI-HA complex resulted in considerable reduction of the tumor growth in C57BL/6 mice. |
| 저자 |
박기태1, Jiang Ge1, 김기수2, 김지석3, 한세광1
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| 소속 |
1포항공과대, 2Dalian Univ., 3Dalian |
| 키워드 |
Hyaluronic acid; Polyethylenimine; Small interfering RNA; LYVE-1 HA receptor; Intracellular gene delivery.
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| E-Mail |
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