화학공학소재연구정보센터
학회 한국고분자학회
학술대회 2018년 가을 (10/10 ~ 10/12, 경주컨벤션센터)
권호 43권 2호
발표분야 의료용 고분자 부문위원회 II
제목 Synthesis and Evaluation of Reduction-responsive Gene Carrier based on chitosan for enhanced Transfection
초록 The aim of this study was to develop a new gene carrier composed of chitosan (CS), cystamine(CA), and PEI. We found that CA and PEI modified chitosan (CSCAP) can be deliver faster gene into nucleus of mammalian cell. Gelretardation assay showed that pDNA was completely condensed by CSCAP, and protected from DNase. Also, Its transfection was enhanced by proton sponge effect with modified PEI, and disulfide bond at modified CA was dissociated by glutathione into cell. In addition, its cellular uptake was investigated by fluorescent microscope. Characterization of CSCAP was analysed by FT-IR, 1H-NMR, DLS and TEM. Moreover, Its cytotoxicity was confirmed by MTT assay. These results suggest that CSCAP can apply as a carrier of gene delivery. This work was supported by National Research Foundation of Korea (NRF) grant funded by the Ministry of Science, ICT & Future Planning (NRF-2016H1D5A1910499).
저자 정경원, 안준혁, 홍웅길, 나재운
소속 순천대
키워드 Chitosan; PEI; Cystamine; Reduction-sensitive; Gene transfection
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