| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | Effects of 1,25(OH)2D Loaded PLGA Scaffolds on in Vivo Bone Formation with Marrow Stromal Cells |
| 초록 | Autologous bone graft is one of the most effective methods to repair various bone defects now, but significant problems such as donor site morbidity and limitation of supply are unresolved. At the present time, tissue engineered bone formation is made using scaffolds, osteogenic growth factors and osteoblasts. Calcitriol is widely used to prevent fractures for people who have risk factors of osteoporosis. In this study, we fabricated sustained release 1,25(OH)2D (calcitriol) PLGA scaffolds, and seeded bone marrow stromal cells (BMSC) onto that to augment bone regenerability. In addition, we used acellular porcine small intestinal submucosa (SIS) sheets for migration of osteoblasts, trapping of osteogenic growth factors and stability of implanted scaffolds. Cylindrical scaffolds were prepared PLGA, NaCl and Calcitriol, which were 5mm in height, 6mm in diameter. BMSC aspirates were obtained from femur of 2-week-old rabbits and then seeded 4ⅹ106 cells/scaffolds right before implantation. SIS sheets were produced by SIS after removing mucosa, muscularis and serosa with EDC as a cross linking agent. We fixed femur of 7-week-old rabbits by external fixators and then implanted cell-scaffolds complex (3 cylinders) after removing mid-shaft about 1.5cm. We covered bone defects with SIS sheets including both defected ends before suturing lateral thigh muscles. Experimental groups were divided into calcitriol loaded, calcitriol unloaded and no implant groups. We evaluated bone defects with X-ray every other week till 8th week postoperatively. Bone union progressed fastest in calcitriol loaded group. Anatomical properties of repaired bone in calcitriol loaded group were similar to intact femur confirmed by 3D-CT in the 24th week. In the early 2nd week, ALP-positive osteoblasts were seen on the inner surface of the SIS sheets. In the 3rd week, numerous collagen fibers of the SIS sheets were embedded in adjacent bone matrix of both fractured ends. Acknowledgement: This work was supported by the Musculoskeletal Bioorgan Center (0405-BO01-0204-0006) grant from KMOHW. References 1. S.J. Lee, D.H. Lee, G. Khang, Y.M. Lee and H.B. Lee, Macromol. Chem. Symp., 15(4), 201 (2002) |
| 저자 | 윤선중1, 박기숙1, 하현정1, 최방실1, 고연경1, 김문석2, 이해방2, 이종문1, 강길선1 |
| 소속 | 1전북대, 2한국화학(연) |
| 키워드 | Calcitriol; SIS; Marrow Stromal cells; Bone tissue engineering |
