| 초록 |
Bioprinting is an emerging technology to produce biomimetic 3-D structures using cell-containing hydrogels as bioink. A number of properties are required for hydrogels to be used for bioink, including biocompatibility, biodegradability, printability and cross-linkability. However, most of hydrogels lack cell-interactive motifs and there have been many challenges to grow cells within the scaffolds into desired tissues. Here, we propose that genetically engineered M13 bacteriophage (phage) has great potential as nano-bioink for printing 3-D cell-laden scaffolds. M13 phages displaying both integrin-binding and calcium-binding domains on their surface were blended with alginate to form hydrogels via Ca2+-mediated crosslinking. In particular, MC3T3-E1 cells within 3-D scaffolds composed of M13 phage and alginate showed higher cell viability, enhanced proliferation and differentiation rate that increased proportionally to concentration of phages in the 3-D matrices as compared with the rates of cells in the scaffolds of pure alginate. |
