| 학회 |
한국고분자학회 |
| 학술대회 |
2006년 봄 (04/06 ~ 04/07, 일산킨텍스) |
| 권호 |
31권 1호 |
| 발표분야 |
광/전 기능 고분자-바이오 융합기술 심포지움 |
| 제목 |
A Magnetic Nanoprobe Technology for Imaging Molecular Interactions Inside Living Human Cells |
| 초록 |
We present a novel technology, "magnetism-based interaction capture (MAGIC)", for molecular target identification based on induced movement of superparamagnetic nanoparticles inside living cells. Transducible fusogenic peptide led to efficient intracellular uptake of superparamagnetic nanoparticles coated with a small molecule of interest. These nanoprobes captured the small molecule’s labeled target protein and were translocated in a direction specified by an external magnetic field. Implementation of the MAGIC principle to genome-wide expression screening successfully identified the protein targets of a drug. Furthermore, we demonstrate that MAGIC technology can be used to monitor signal-dependent modification and multiple interactions of proteins. Our nanotechnology will be useful for probing a variety of molecular (e.g. drug-protein & protein-protein) interactions in single live cells. |
| 저자 |
김태국 |
| 소속 |
KAIST |
| 키워드 |
Molecular Interactions; Nanoprobe; Live Technology
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| E-Mail |
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