| 학회 | 한국공업화학회 |
| 학술대회 | 2015년 봄 (04/29 ~ 05/01, BEXCO (부산)) |
| 권호 | 19권 1호 |
| 발표분야 | (콜로이드계면화학) 나노소재 기술에 있어서 콜로이드 공학의 응용 (중앙대 임경희 교수 정년 퇴임 기념) |
| 제목 | Protein-based Organic/Inorganic Hybrid Nanostructures for In vivo Theranostic Applications |
| 초록 | In this talk I will report on serum-stable hybrid nanocapsules of human serum albumin (HAS) cross-linked with six-arm-branched polyethylene glycol (PEG) as a cross-linker. Human serum albumin (HSA) is highly abundant in the blood plasma and transports fatty acids, hormones, bilirubin, and other small molecules. It has a long half-life in serum (about 20 days), making the protein attractive for in vivo systemic delivery of therapeutic agents. However, non-specific binding of molecules to the hydrophobic patches of HSA often leads to undesirable aggregation because of uncontrolled changes in the interfacial properties of the protein. To solve this problem, we employed six-arm-branched PEG as a cross-linker. The HSA/PEG hybrid nanocapsules are prepared by emulsifying an organic solution of amine-reactive six-arm-branched PEG into an aqueous solution of HSA. The successful formation of nanocapsules are kinetically determined by several processes: diffusion of PEG molecules out of the organic phase, adsorption of HSA onto the emulsion surfaces, and cross-linking reactions between PEG and HSA at the organic/aqueous interface. The nanocapsules are employed as in vivo delivery carriers for paclitaxel and inorganic nanocrystals. In several types of cells, the surface modification of nanocapsules with a cell-penetrating peptide or cancer-targeting molecule greatly facilitates cellular uptake and apoptosis-inducing effects of paclitaxel. Furthermore, the targeted anti-tumor activities of the paclitaxel-loaded nanocapsules in a mouse tumor model suggest that the shell cross-linked nanocapsules are very promising oil-free nanoscale delivery vehicles for water-insoluble anti-cancer agents. In addition, cadmium-free CuInS/ZnS quantum dots, and monodisperse iron oxide nanocrystals are encapsulated within the shell as an imaging probe. I will discuss physicochemical properties of the prepared hybrid nanocapsules in relation with their cytotoxicities and in vivo destinations. This study demonstrates that the HSA/PEG hybrid nanocapsules can be utilized as a biocompatible, cell-targeting platform for in vivo systemic delivery of various therapeutic and imaging agent. This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (NRF-2013R1A1A1009626). |
| 저자 | 남윤성 |
| 소속 | 한국과학기술원 |
| 키워드 | nanoparticles; albumin; hybrid; theranostic; delivery |
