화학공학소재연구정보센터

Protein Expression and Purification

Protein Expression and Purification, Vol.47, No.1 Entire volume, number list
ISSN: 1046-5928 (Print) 

In this Issue (41 articles)

1 - 9 Expression, purification, and characterization of a new heterotetramer structure of leucyl-tRNA synthetase from Aquifex aeolicus in Escherichia coli
Olieric N, Bey G, Nierengarten H, Wang ED, Moras D, Eriani G, Cavarelli J
10 - 15 An improved expression plasmid for affinity purification of Staphylococcus aureus gyrase A subunit
Strahilevitz J, Onodera Y, Hooper DC
16 - 24 Automated purification of recombinant proteins: Combining high-throughput with high yield
Lin CT, Moore PA, Auberry DL, Landorf EV, Peppler T, Victry KD, Collart FR, Kery V
25 - 35 Expression and purification of recombinant human receptor for advanced glycation endproducts in Escherichia coli
Wilton R, Yousef MA, Saxena P, Szpunar M, Stevens FJ
36 - 44 Construction, expression, and purification of HIV-TAT-survivin (T34A) mutant: A pro-apoptosis protein in Escherichia coli
Ma XY, Zheng WY, Wei DZ, Ma YS, Wang TW, Wang JZ, Liu QH, Yang SL
45 - 51 A novel "reverse screening" to identify refolding additives for activin-A
Ejima D, Ono K, Tsumoto K, Arakawa T, Eto Y
52 - 59 Cloning and expression of superoxide dismutase from Mycobacterium bovis BCG
Kang SK, Chung TW, Lee JH, Kim CH
60 - 67 Purification of novel UBAP1 protein and its decreased expression on nasopharyngeal carcinoma tissue microarray
Xiao BY, Fan SQ, Zeng ZY, Xiong W, Cao L, Yang YX, Li WF, Wang R, Tang K, Qian J, Shen SR, Li XL, Li GY
68 - 73 Production of bioactive human granulocyte-colony stimulating factor in transgenic rice cell suspension cultures
Hong SY, Kwon TH, Jang YS, Kim SH, Yang MS
74 - 81 Expression, purification, and kinetic characterization of recombinant rat cysteine dioxygenase, a non-heme metalloenzyme necessary for regulation of cellular cysteine levels
Simmons CR, Hirschberger LL, Machi MS, Stipanuk MH
82 - 92 Expression and purification of a single-chain variable fragment antibody derived from a polyol-responsive monoclonal antibody
Lamberski JA, Thompson NE, Burgess RR
93 - 98 Expression of recombinant kringle 1-5 domains of human plasminogen by a prokaryote expression system
Hou WH, Fang T, Chai YR, Wang TY, Wang JM, Xue LX
99 - 109 Synthesis and assembly of an a uvanted Porphyromonas gingivalis is fimbrial antigen fusion protein in plants
Shin EA, Lee JY, Kim TG, Park YK, Langridge WHR
110 - 117 Fusion expression of bovine lactoferricin in Escherichia coli
Feng X, Wang J, Shan A, Teng D, Yang Y, Yao Y, Yang G, Shao Y, Liu S, Zhang F
118 - 127 An extracellular exo-beta-(1,3)-glucanase from Pichia pastoris: Purification, characterization, molecular cloning, and functional expression
Xu ZW, Shih MC, Poulton JE
128 - 136 Large-scale preparation of biologically active mouse and rat leptins and their L39A/D40A/F41A muteins which act as potent antagonists
Salomon G, Niv-Spector L, Gussakovsky EE, Gertler A
137 - 143 Purification and characterization of a soluble form of the recombinant human galactose-beta 1,3-glucuronosyltransferase I expressed in the yeast Pichia pastoris
Lattard V, Fondeur-Gelinotte M, Gulberti S, Jacquinet JC, Boudrant J, Netter P, Magdalou J, Ouzzine M, Fournel-Gigleux S
144 - 151 Efficient solubilization, activation, and purification of recombinant Cry45Aa of Bacillus thuringiensis expressed as inclusion bodies in Escherichia coli
Okumura S, Saitoh H, Wasano N, Katayama H, Higuchi K, Mizuki E, Inouye K
152 - 161 Challenges in the expression of disulfide bonded, threonine-rich antifreeze proteins in bacteria and yeast
Tyshenko MG, d'Anjou M, Davies PL, Daugulis AJ, Walker VK
162 - 170 Expression and characterization of recombinant tyramine beta-monooxygenase from Drosophila: A monomeric copper-containing hydroxylase
Gray EE, Small SN, McGuirl MA
171 - 178 Over-expression of a mammalian small conductance calcium-activated K+ channel in Pichia pastoris: Effects of trafficking signals and subunit fusions
Licata L, Haase W, Eckhardt-Strelau L, Parcej DN
179 - 185 In vitro refolding of recombinant human free secretory component using equilibrium gradient dialysis
Prinsloo E, Oosthuizen V, Muramoto K, Naude RJ
186 - 193 Purification and cytotoxic properties of Bacillus cereus hemolysin II
Andreeva ZI, Nesterenko VF, Yurkov IS, Budarina ZI, Sineva EV, Solonin AS
194 - 202 Recombinant production of anti-HIV protein, griffithsin, by auto-induction in a fermentor culture
Giomarelli B, Schumacher KM, Taylor TE, Sowder RC, Hartley JL, McMahon JB, Mori T
203 - 209 Functional expression of single-chain variable fragment antibody against c-Met in the cytoplasm of Escherichia coli
Heo MA, Kim SH, Kim SY, Kim YJ, Chung JH, Oh MK, Lee SG
210 - 216 Expression and purification of the intact cytoplasmic domain of the human ephrin receptor A2 tyrosine kinase in Escherichia coli
Zabell KM, Laurence JS, Kinch MS, Knapp DW, Stauffacher CV
217 - 224 A set of ligation-independent expression vectors for co-expression of proteins in Escherichia coli
Chanda PK, Edris WA, Kennedy JD
225 - 233 Expression and purification of the subunits of human translational initiation factor 2 (eIF2): Phosphorylation of eIF2 alpha and beta
Suragani RNVS, Ghosh S, Ehtesham NZ, Ramaiah KVA
234 - 240 Efficient high level expression of peptides and proteins as fusion proteins with the N-terminal domain of L9: Application to the villin headpiece helical subdomain
Bi Y, Tang YF, Raleigh DP, Cho JH
241 - 248 Heterologous expression of dodecaheme "nanowire" cytochromes c from Geobacter sulfurreducens
Londer YY, Pokkuluri PR, Orshonsky V, Orshonsky L, Schiffer M
249 - 257 Codon optimization, expression, and characterization of an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris
Hu SY, Li LW, Qiao JJ, Guo YJ, Cheng LS, Liu J
258 - 263 Improving protein solubility: The use of the Escherichia coli dihydrofolate reductase gene as a fusion reporter
Liu JW, Boucher Y, Stokes HW, Ollis DL
264 - 272 Coexpression of the subunits of T7 DNA polymerase from an artificial operon allows one-step purification of active gp5/Trx complex
Chiu J, Tillett D, March PE
273 - 280 Purification and biochemical characterization of a monomeric form of papaya mosaic potexvirus coat protein
Lecours K, Tremblay MH, Gagne MEL, Gagne SM, Leclerc D
281 - 288 Quantitative evaluation of mammalian skeletal muscle as a heterologous protein expression system
DiFranco M, Neco P, Capote J, Meera P, Vergara JL
289 - 295 Expression of compstatin in Escherichia coli: Incorporation of unnatural amino acids enhances its activity
Katragadda M, Lambris JD
296 - 302 Large-scale purification and characterization of human parathyroid hormone-1 receptor stably expressed in HEK293S GnTI(-) cells
Gan L, Alexander JM, Wittelsberger A, Thomas B, Rosenblatt M
303 - 310 Gi/o proteins: Expression for direct activation enquiry
Marinelli LD, Pacini A, Toscano A, Fortini M, Berti D, Ghelardini C, Galeotti N, Baglioni P, Bartolini A
311 - 318 Novel protein purification system utilizing an N-terminal fusion protein and a caspase-3 cleavable linker
Feeney B, Soderblom EJ, Goshe MB, Clark AC
319 - 328 A novel recombinant multiepitope protein as a hepatitis C diagnostic intermediate of high sensitivity and specificity
Dipti CA, Jain SK, Navin K
329 - 339 Cloning, expression, purification, and properties of a putative plasma membrane hexokinase from Solanum chacoense
Claeyssen E, Wally O, Matton DP, Morse D, Rivoal J