Protein Expression and Purification

Protein Expression and Purification, Vol.48, No.1 Entire volume, number list

ISSN: 1046-5928 (Print)

In this Issue (22 articles)

1 - 13	Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins Arnau J, Lauritzen C, Petersen GE, Pedersen J
14 - 23	Development of the system ensuring a high-level expression of hepatitis C virus nonstructural NS5B and NS5A proteins Ivanov AV, Korovina AN, Tunitskaya VL, Kostyuk DA, Rechinsky VO, Kukhanova MK, Kochetkov SN
24 - 27	Site-directed PEGylation of human basic fibroblast growth factor Wu XP, Li XK, Zeng YY, Zheng Q, Wu SX
28 - 31	Protein expression enhancement in efflux-deleted mutant bacteria Le TT, Guet OC, Cluzel P
32 - 42	Expression in yeast and purification of a membrane protein, SERCA1a, using a biotinylated acceptor domain Jidenko M, Lenoir G, Fuentes JM, le Maire M, Jaxel C
43 - 48	Production of a recombinantly expressed laminin fragment by HEK293-EBNA cells cultured in suspension in a dialysis-based bioreactor Belin V, Rousselle P
49 - 55	Overexpression and characterization of Wzz of Escherichia coli O86 : H2 Guo H, Lokko K, Zhang Y, Yi W, Wu Z, Wang PG
56 - 60	High-level bacterial expression and purification of human SirT2 protein for NMR studies Sun CH, Song DY, Marcotte PA, Richardson PL, Hajduk PJ
61 - 68	The production of cleaved, trimeric human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein vaccine antigens and infectious pseudoviruses using linear polyethylenimine as a transfection reagent Kirschner M, Monrose V, Paluch M, Techodamrongsin N, Rethwilm A, Moore JP
69 - 73	Soluble expression of a strong thrombolytic pro-urokinase mutant in Escherichia coli Lin J, Yang XY, Deng RQ, Yu BG, Lai HJ, Sun WL, Wu WY
74 - 80	Expression and purification of the synthetic preS1 gene of Hepatitis B Virus with preferred Escherichia coli codon preference Qian BJ, Shen HF, Xiong JL, Chen L, Zhang L, Jia J, Wang Y, Zhang ZC, Yuan Z, Cao KM, Zhang DB
81 - 89	cDNA cloning, expression, purification, distribution, and characterization of biologically active canine alanine aminotransferase-1 Rajamohan F, Nelms L, Joslin DL, Lu B, Reagan WJ, Lawton M
90 - 97	Cloning, high yield over-expression, purification, and characterization of CG18594, a new PEBP/RKIP family member from Drosophila melanogaster Rautureau G, Jouvensal L, Decoville M, Locker D, Vovelle FO, Schoentgen FO
98 - 103	Cloning, expression, purification, and characterization of Leishmania major dihydroorotate dehydrogenase Feliciano PR, Cordeiro AT, Costa AJ, Nonato MC
104 - 111	Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli Kiraly O, Guan L, Szepessy E, Toth M, Kukor Z, Sahin-Toth M
112 - 117	Preparation of alpha-synuclein fibrils for solid-state NMR: Expression, purification, and incubation of wild-type and mutant forms Kloepper KD, Woods WS, Winter KA, George JM, Rienstra CM
118 - 125	Cloning, expression, purification, and characterization of Vibrio cholerae transcriptional activator, HlyU Saha RP, Basu G, Chakrabarti P
126 - 133	A simplified method for purification of recombinant soluble DnaA proteins Zawilak-Pawlik AM, Kois A, Zakrzewska-Czerwinska J
134 - 141	Histidine tag fusion increases expression levels of active recombinant amelogenin in Escherichia coli Svensson J, Andersson C, Reseland JE, Lyngstadaas P, Bulow L
142 - 150	Two methods for improved purification of full-length mammalian proteins that have poor expression and/or solubility using standard Escherichia coli procedures Geng J, Carstens RP
151 - 159	Refolding, purification, and characterization of human and murine RegIII proteins expressed in Escherichia coli Cash HL, Whitham CV, Hooper LV
160 - 166	Long stretches of sequential and identical serine or alanine codons are compatible with an efficient full-length protein expression in Escherichia coli Bonomi G