Protein Expression and Purification

Protein Expression and Purification, Vol.77, No.1 Entire volume, number list

ISSN: 1046-5928 (Print)

In this Issue (18 articles)

1 - 8	High-throughput construction of expression system using yeast Pichia pastoris, and its application to membrane proteins Mizutani K, Yoshioka S, Mizutani Y, Iwata S, Mikami B
9 - 19	Model based optimization of the fed-batch production of a highly active transglutaminase variant in Escherichia coli Sommer C, Volk N, Pietzsch M
20 - 25	Expression of Apostichopus japonicus lysozyme in the methylotrophic yeast Pichia pastoris Wang TT, Xu YP, Liu WJ, Sun YX, Jin LJ
26 - 33	The epitope for the polyol-responsive monoclonal antibody 8RB13 is in the flap-domain of the beta-subunit of bacterial RNA polymerase and can be used as an epitope tag for immunoaffinity chromatography Stalder ES, Nagy LH, Batalla P, Arthur TM, Thompson NE, Burgess RR
34 - 45	New ligation-independent cloning vectors compatible with a high-throughput platform for parallel construct expression evaluation using baculovirus-infected insect cells Brown WC, DelProposto J, Rubin JR, Lamiman K, Carless J, Smith JL
46 - 52	High-level soluble expression of recombinant human manganese superoxide dismutase in Escherichia coli, and its effects on proliferation of the leukemia cell Wang F, Mei S, Yang WJ, Huang LY
53 - 61	The substrate specificity of Metarhizium anisopliae and Bos taurus carboxypeptidases A: Insights into their use as tools for the removal of affinity tags Austin BP, Tozser J, Bagossi P, Tropea JE, Waugh DS
62 - 67	Expression and characterization of recombinant Locusta migratoria manilensis acetylcholinesterase 1 in Pichia pastoris Zhou XX, Xia YX
68 - 74	Refolding single-chain antibody (scFv) using lauroyl-L-glutamate as a solubilization detergent and arginine as a refolding additive Kudou M, Ejima D, Sato H, Yumioka R, Arakawa T, Tsumoto K
75 - 79	FhuA deletion variant Delta 1-159 overexpression in inclusion bodies and refolding with Polyethylene-Poly(ethylene glycol) diblock copolymer Dworeck T, Petri AK, Muhammad N, Fioroni M, Schwaneberg U
80 - 85	Expression of the HSV-1 capsid protein VP19C in Escherichia coli: A single amino acid change overcomes an expression block of the full-length polypeptide Henson BW, Johnson N, Bera A, Okoye ME, Desai KV, Desai PJ
86 - 91	Expression and purification of human FROUNT, a common cytosolic regulator of CCR2 and CCR5 Esaki K, Terashima Y, Toda E, Yoshinaga S, Araki N, Matsushima K, Terasawa H
92 - 97	Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems Dadashipour M, Fukuta Y, Asano Y
98 - 103	Expression, purification, and refolding of biologically active Acinetobacter baumannii OmpA from Escherichia coli inclusion bodies McConnell MJ, Pachon J
104 - 111	Screening of genetic parameters for soluble protein expression in Escherichia coli Vernet E, Kotzsch A, Voldborg B, Sundstrom M
112 - 117	Heterologous expression of Translocated promoter region protein, Tpr, identified as a transcription factor from Rattus norvegicus Agarwal S, Yadav SK, Dixit A
118 - 123	Identification of TMEM45B as a protein clearly showing thermal aggregation in SDS-PAGE gels and dissection of its amino acid sequence responsible for this aggregation Okada N, Yamamoto T, Watanabe M, Yoshimura Y, Obana E, Yamazaki N, Kawazoe K, Shinohara Y, Minakuchi K
124 - 130	A chimera of green fluorescent protein with single chain variable fragment antibody against ginsenosides for fluorescence-linked immunosorbent assay Sakamoto S, Tanizaki Y, Pongkitwitoon B, Tanaka H, Morimoto S