Protein Expression and Purification

Protein Expression and Purification, Vol.82, No.1 Entire volume, number list

ISSN: 1046-5928 (Print)

In this Issue (38 articles)

1 - 5	A simple and efficient protocol for the production of recombinant cathepsin V and other cysteine cathepsins in soluble form in Escherichia coli Novinec M, Pavsic M, Lenarcic B
6 - 10	Expression of a biologically-active conotoxin PrIIIE in Escherichia coli Hernandez-Cuebas LM, White MM
11 - 19	Production, purification and characterization of mouse monoclonal antibodies against human mitochondrial transcription termination factor 2 (MTERF2) Xiong W, Huang WW, Jiao Y, Ma J, Yu M, Ma MX, Wu H, Tan DY
20 - 25	Expression and immunoaffinity purification of recombinant dengue virus 2 NS1 protein as a cleavable SUMOstar fusion Rozen-Gagnon K, Moreland NJ, Ruedl C, Vasudevan SG
26 - 31	RNA secondary structure and in vitro translation efficiency Freischmidt A, Liss M, Wagner R, Kalbitzer HR, Horn G
32 - 36	Optimized refolding and characterization of active C-terminal ADAMTS-18 fragment from inclusion bodies of Escherichia coli Dang SY, Hong T, Bu DW, Tang J, Fan J, Zhang W
37 - 44	Optimized production of scygonadin in Pichia pastoris and analysis of its antimicrobial and antiviral activities Peng H, Liu HP, Chen B, Hao H, Wang KJ
45 - 54	Expression, purification and some properties of fluorescent chimeras of human small heat shock proteins Datskevich PN, Mymrikov EV, Sluchanko NN, Shemetov AA, Sudnitsyna MV, Gusev NB
55 - 60	One-step purification of a functional, constitutively activated form of visual arrestin Huang L, Mao X, Abdulaev NG, Ngo T, Liu W, Ridge KD
61 - 69	Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells Machida K, Masutani M, Kobayashi T, Mikami S, Nishino Y, Miyazawa A, Imataka H
70 - 74	Expression and purification of non-N-glycosylated porcine interleukin 3 in yeast Pichia pastoris Hermanrud CE, Pathiraja V, Matar A, Duran-Struuck R, Crepeau RL, Srinivasan S, Sachs DH, Huang CA, Wang ZR
75 - 82	Thermolabile antifreeze protein produced in Escherichia coli for structural analysis Lin FH, Sun TJ, Fletcher GL, Davies PL
83 - 89	Production of recombinant Rhizopus oryzae lipase by the yeast Yarrowia lipolytica results in increased enzymatic thermostability Yuzbashev TV, Yuzbasheva EY, Vibornaya TV, Sobolevskaya TI, Laptev IA, Gavrikov AV, Sineoky SP
90 - 96	Modular cloning and protein expression of long, repetitive resilin-based proteins Renner JN, Kim Y, Cherry KM, Liu JC
97 - 105	Purification and characterization of riproximin from Ximenia americana fruit kernels Bayer H, Ey N, Wattenberg A, Voss C, Berger MR
106 - 115	Efficient and versatile one-step affinity purification of in vivo biotinylated proteins: Expression, characterization and structure analysis of recombinant human glutamate carboxypeptidase II Tykvart J, Sacha P, Barinka C, Knedlik T, Starkova J, Lubkowski J, Konvalinka J
116 - 124	Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3) Austin BP, Waugh DS
125 - 131	Expression analysis of human p-secretase in transgenic tomato fruits Kim HS, Youm JW, Moon KB, Ha JH, Kim YH, Joung H, Jeon JH
132 - 137	Caenorhabditis elegans Hsp70-1 expresses highly in bacteria, is sufficiently soluble, and has a catalytic constant similar to Hsc70 and BiP Odunuga OO, Bollinger SA, Choi KH, Polvadore EI
138 - 143	Cloning, expression, purification, and characterization of a glutamate-specific endopeptidase from Bacillus licheniformis Ye W, Liu JW, Wang HY, Wang JF, Wang XN
144 - 149	Expression, purification and characterization of recombinant human serine proteinase inhibitor Kazal-type 6 (SPINK6) in Pichia pastoris Lu HR, Huang JJ, Li GD, Ge KK, Wu HY, Huang QS
150 - 154	Cloning, expression and characterization of the recombinant Yersinia pseudotuberculosis L-asparaginase Pokrovskaya MV, Aleksandrova SS, Pokrovsky VS, Omeljanjuk NM, Borisova AA, Anisimova NY, Sokolov NN
155 - 161	Efficient expression of recombinant soluble human Fc gamma RI in mammalian cells and its characterization Asaoka Y, Tanaka T, Tsumoto K, Tomita M, Ide T
162 - 167	Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation Li YF, Sousa R
168 - 173	Methyl-beta-cyclodextrin is a useful compound for extraction and purification of prenylated enzymes from the retinal disc membrane Saito N, Ishida Y, Seno K, Hayashi F
174 - 178	Ubiquitin-intein and SUMO2-intein fusion systems for enhanced protein production and purification Wang ZY, Li N, Wang YY, Wu YP, Mu TY, Zheng Y, Huang LQ, Fang XX
179 - 185	Glycosylations and truncations of functional cereal phytases expressed and secreted by Pichia pastoris documented by mass spectrometry Dionisio G, Jorgensen M, Welinder KG, Brinch-Pedersen H
186 - 191	Cloning, soluble expression, rapid purification and characterization of human Cofilin1 Lu JX, Xiang YF, Zhang JX, Ju HQ, Chen ZP, Wang QL, Chen W, Peng XL, Han B, Wang YF
192 - 196	Comparison of two expression systems using COS7 cells and yeast cells for expression of heart/muscle-type carnitine palmitoyltransferase 1 Hada T, Kato Y, Obana E, Yamamoto A, Yamazaki N, Hashimoto M, Yamamoto T, Shinohara Y
197 - 204	Overproduction of anti-Tn antibody MLS128 single-chain Fv fragment in Escherichia coli cytoplasm using a novel pCold-PDI vector Subedi GP, Satoh T, Hanashima S, Ikeda A, Nakada H, Sato R, Mizuno M, Yuasa N, Fujita-Yamaguchi Y, Yamaguchi Y
205 - 211	Expression and purification of recombinant human Mig in Escherichia coli and its comparison with murine Mig Qian L, Zhu SY, Shen JQ, Han XD, Gao J, Wu MY, Yu Y, Lu HL, Han W
212 - 217	Sequencing, cloning and high-yield expression of a fungal beta-N-acetylhexosaminidase in Pichia pastoris Slamova K, Bojarova P, Gerstorferova D, Fliedrova B, Hofmeisterova J, Fiala M, Pompach P, Kren V
218 - 225	Expression screening of membrane proteins with cell-free protein synthesis Isaksson L, Enberg J, Neutze R, Karlsson BG, Pedersen A
226 - 231	Tobacco etch virus protease retains its activity in various buffers and in the presence of diverse additives Sun CS, Liang JQ, Shi R, Gao XN, Zhang RJ, Hong FL, Yuan QH, Wang SB
232 - 239	Preparation of human vascular endothelial growth factor-D for structural and preclinical therapeutic studies Davydova N, Streltsov VA, Roufail S, Lovrecz GO, Stacker SA, Adams TE, Achen MG
240 - 251	Production of disulfide-bonded proteins in Escherichia coli Berkmen M
252 - 252	Recombinant production of antimicrobial peptides in Escherichia coli: A review (vol 80, pg 206, 2011) Li YF
253 - 253	High level expression, purification and characterization of active fusion human C1q and tumor necrosis factor related protein 2 (hCTRP2) in Escherichia coli (vol 79, pg 1, 2011) Li HB, Gao XF, Zhou Y, Li N, Ge CZ, Hui XY, Wang Y, Xu AM, Jin SG, Wu DH