Sepiolite was used as support for ruthenium catalyst. Non-promoted Ru/Sep is proved to be effective for ammonia synthesis. The catalyst was characterized by XRD, XPS and nitrogen adsorption isotherms. Ru is reduced completely and is present in few oxidation states on the support. Using non-aqueous solvent instead of water in the impregnation process on basic support, the Ru dispersion could be increased. Chlorine-free Ruthenium catalyst was prepared using RuCl3 by washing with ammonia solution.