Sucrose is one of the abundant bio-mass on the earth, which can be easily processed for the production of edible nondigestive sweetener fructo-oligosaccharide(s) using an enzyme fructosyl transferase. The physicochemical properties of fructosyl transferase can be exploitable for the industrial operations. The direction of efforts was to study the factors like heavily glycosylated nature of enzyme, presence of sugars like sucrose, glucose, fructose and their mixtures responsible for stability of the fructosyl transferase at elevated temperatures. Effects of different metals on enzyme activity were also studied.