Commercial uricase has been immobilized covalently onto alkylamine glass beads affixed on a plastic strip with a conjugation yield of 87mg/g and 11.5% retention of initial activity of free enzyme. Maximum activity of immobilized enzyme was attained at pH 8.8, when incubated at 40degreesC for 20 min. K-m for uric acid and V-max of immobilized enzyme were 0.15mM and 0.68mumoles H2O2/min respectively. A method for uric acid determination in serum and urine was developed using the strip bound enzyme. The method is based on the measurement of H2O2 generated from uric acid by using a colour reaction consisting of 4-aminophenazone, p-hydroxybenzoic acid and horseradish peroxidase as chromogenic system. The minimum detection limit of the method was 1.68mg/dl and recovery of added uric acid in urine was 90% The coefficient of variation (CV) were <5 and <2% for within batch and between batch respectively. The immobilized enzyme lost 50% of its initial activity after its regular uses for over 3 weeks, when stored in 0.05M glycine NaOH buffer, pH 8.8 at 4degreesC.