Baker's yeast was disintegrated in a bead mill. Concentration of the suspension and impeller rotations were changed. The amount of released protein and absorbency at the wavelength 260 nm were measured. With an increase of the suspension concentration a change in the character of the process from linear (for a very low concentration) to non-linear was observed. Conditions of the release of intracellular components were considered to depend significantly on the concentration of biomass and cell disintegration.