In this study, we have demonstrated the use of piezoelectric inkjet printing to fabricate microscale patterns of Vetbond (R) n-butyl cyanoacrylate tissue adhesive. Optical microscopy, atomic force microscopy, nanoindentation, and a cell viability assay were used to examine the structural, mechanical, and biological properties of microscale cyanoacrylate patterns. The ability to rapidly fabricate microscale patterns of medical and veterinary adhesives will enable reduced bond lines between tissues, improved tissue integrity, and reduced toxicity. We envision that piezoelectric inkjet deposition of cyanoacrylates and other medical adhesives may be used to enhance wound repair in microvascular surgery.