In this work we have studied the effect of adhesion forces on the mechanical parameters between a spherical indenter and human skin surface with an indentation test. To take into account the effect of adhesion on Hertzian contact radius, pressure and strain, a theory of adhesion contact, like that of Johnson, Kendall and Roberts (JKR) or Derjaguin, Muller and Toporov (DMT) must be used. These theories correct the errors induced by the adhesion forces on contact parameters. The change in skin surface lipid film during hydration by water is assessed by analyzing the evolution of the adhesion energy and skin stiffness.