The densities and speeds of sound of five fatty acid methyl esters (FAMEs) were measured at approximately 83 kPa (atmospheric pressure in Boulder, CO) at temperatures from (278.15 to 338.15) K. The FAMEs measured were the five primary components of biodiesel fuel, namely, methyl palmitate, methyl stearate, methyl oleate, methyl linoleate, and methyl linolenate. The densities and speeds of sound were measured concurrently with a commercial instrument equipped with a vibrating U-tube densimeter and pulse-echo speed of sound measurement cell. The densities of the FAMEs agreed with and expanded upon limited available literature values. The speed of sound measurements provide much needed experimental data for equation of state development.