In this paper, sucrose sensor utilizing two enzymes, invertase (INV) and glucose oxidase (GOD), was developed without utilizing pig kidney mutarotase, which catalyses mutarotation reaction (the conversion of alpha-D-glucose to beta-D-glucose). The GOD electrode and the GOD+INV electrode were made by immobilizing enzymes on platinum electrode with glutaraldehyde. The effects of phosphate ion concentration and temperature on sucrose measurements were studied. In respect of responsivity and durability of enzymes, the optimum temperature and phosphate ion concentration was found to be 308 K and 0.5 mol.L(-1), respectively. The concentrations of sucrose and glucose were measured by using two electrodes, GOD electrode and GOD+INV electrode. This study showed that sucrose concentration can be determined from the deviation of the current of GOD+INV electrode from that of GOD electrode.