The adsorption of squalene, as model molecule of polyisoprene, on a silica surface, in anhydrous heptane, has been studied using inverse liquid chromatographic analysis. Unlike HPLC supports, the efficiency of the chromatographic column filled with a silica designed for rubber applications is low. Then the front boundaries of a non-adsorbed molecule, like cyclohexane, was taken as reference for the calculation of the desorption isotherms. Changing experimental parameters, which influence the column efficiency, the validity of this approach was carefully checked. No significant influence of the studied experimental parameters was observed and the measurement precision of both monolayercapacity(Q(0)) und Langmuir's constant (B) was better than 6%. The same ILC analysis was extended to three polymer probes: a low polyisoprene telomer and 2 polybutadiene having a high molecular weight. It was observed that the polymer probes behave differently from the squalene: the polymer adsorbs strongly irreversibly and size exclusion effects occur due to the first adsorbed layers.