Films on the oxygenase activity of indigenous microflora in oil-polluted soils having an oil concentration of 50 g/kg was studied. The oil utilization in experiments was 30-35 g/kg of soil versus 8-13 g/kg in the control. The IR spectroscopic determination of residual petroleum hydrocarbons showed the appearance of absorption bands at 1710 and 1600 cm(-1), as well as an increase in the spectral coefficients C-1, C-2, A(1), A(2), and A(2) by a factor 1.5-3 and a decrease in C-3 and A(3) by a factor 1.5-2.5, thus indicating intense oil oxidation in the presence of photoluminescent films. Chromatographic analysis data corroborated the occurrence of intense biodegradation of acyclic saturated hydrocarbons. In experimental samples, light C-9-C-14 hydrocarbons completely disappeared and the concentration of higher C-15-C-28 hydrocarbons decreased by 70-80%. The oil biodegradation factor defined as the ratio of total isoalkanes (pristane + phytane) to total n-alkanes (C-17 + C-18) increases 5-6-fold when the photoluminescent films are used.