The recognition ability of molecularly imprinted polymers (MIPs) is largely governed by factors such as polymerization conditions, nature of crosslinking agents, and degree of crosslinking. In addition to these factors, monomers could also influence the ability of the MIPs to recognize the print molecules. MIPs based on 2-hydroxy ethyl methacrylate and N-vinyl pyrolidone imprinted for cholesterol and testosterone are prepared. The results show that the recognition ability strongly depends on the characteristics of monomer also, in addition to the nature of the print molecule.